Developing a Screening Pipeline for the Identification of Phosphorus-Solubilizing Bacteria
By Juan Quijia Pillajo, Sachin Naik, and Michelle Jones Phosphorus (P) is an essential nutrient with low availability for plant uptake. High media pH levels can cause the formation of insoluble compounds with calcium (Ca). Phosphorus solubilizing bacteria (PSB) can enhance P availability for plant uptake by producing organic acids to acidify the rhizosphere. The identification of PSBs requires both in-vitro and in-planta evaluations. In-vitro protocols allow for the screening of extensive bacterial collections, but the number of bacterial isolates that can be tested in the greenhouse is limited by the amount of space and time required to evaluate the plant responses to PSB inoculation. Digital phenotyping is an emerging tool for high throughput and precise evaluation of plant performance…
