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Efficient Release Strategies for Aphid Natural Enemies in Flower Crops Progress Report — June 1998

Date:7/1/98

Title of Project: Efficient release strategies of natural enemies

in flower crops.

Institution where work is being conducted: Texas A&M

Amount of Endowment Grant: $35,000

Covering Period: 7/97 to 7/98

Anticipated Date of Project Completion /Final Report:

6/30/99

Individual(s) Conducting Project:

(List Project Leader First)

Dr. Kevin Heinz

Telephone Number:

Efficient release strategies of natural

enemies in flower crops

Report Content:

Documentation of the Buildup and Distribution of Aphid Outbreaks in

Chrysanthemums. During the

early stages of an outbreak, aphids form small clumps on individual

plants within chrysanthemum greenhouses. These initial outbreaks

quickly become serious problems because aphid populations quadruple in

size daily when occurring on healthy plants. In addition, results

conducted by my laboratory in Texas A&M University greenhouses demonstrated

that green peach aphids can spread over an area of 120 ft’ per day after

infesting an single potted chrysanthemum. Hence, aphids may be present

for a long period of time within a greenhouse and have the opportunity

to reproduce and spread throughout the crop before being noticed.

These abundance and distribution data formed the basis for the development

of optimal natural enemy releases strategies for biological aphid control

(outlined below), and have been used to develop an aphid sampling plan

that can be used in conjuction with existing and developing sampling plans

for leafminers, spider mites, whitefly, and thrips.

Basic Studies on Aphid Natural Enemies. For predators and parasitoids

to provide successful aphid biological, these natural enemies must locate

and consume aphid patches when they are relatively scarce before the aphids

infest the entire greenhouse. Therefore, growers should only use

the most effective predators and parasitoids, and release them in such

a manner whereby they can locate and kill aphids at a sufficiently high

rate.

Determining exactly how natural enemies respond to aphid patches as

they change in time and space requires long and tedious experiments.

After completing a set of intensive studies, my laboratory discovered the

limitations to obtaining biological aphid control by releasing predators

and parasitoids.Green lacewing larvae, used as a model predator, were found

to be incapable of navigating between potted chrysanthmums placed atop

solid benches. Although lacewing larvae voraciously consume aphids

once discovered, successful biological control requires placement of lacewing

larvae onto each individual plant infested with aphids.

By comparison, studies with the parasitoid wasp A. colemani demonstrated

that it could spread over an area of 147 ft’ per day after being released

from a single potted chrysanthemum. From these results, my lab determined

that the most effective biological aphid control could be obtained by releasing

A. colemani from points no greater than 12 feet apart within a potted chrysanthemum

greenhouse.

Knowing How to Release Natural Enemies Makes a Difference. Greenhouse

trials were conducted to test the influence of A. colemani release strategies

on their ability to biologically control green peach aphids in research

greenhouses. Wasps were released at the rate of three per pot per

week from 4 points, 12 feet apart or from one central point within 1200

ft’ greenhouses filled with potted chrysanthemums. Additionally,

each greenhouse contained a screened cage, which covered a bench of chrysanthemums,

and prevented wasps from accessing aphid infested plants. Comparisons

between aphids densities within the cages to those outside the cages (into

which A. colemani were released) provided an experimental method for assessing

the impact of parasitoid releases. At the beginning of the trial,

every third pot within the greenhouse was infested with 3 green peach aphids.

Both A. colemani treatments (released from I or 4 points) yielded significant

aphid suppression. Densities in the cages from which wasps were excluded

exceeded 5,000 aphids per plant by week 6 of each of the trials.

Aphid densities climbed to 27.5 per plant at week 10 in greenhouses where

wasps were released from one central point. By comparison, aphid

densities reached a maximum of 5.8 per plant at week 3 in greenhouses where

wasps were released from four points.

Harvest quality of the potted chrysanthemums at the completion of each

trial were determined by 14 horticulture and entomology faculty, staff,

and students who judged representative samples of pots from each treatment.

Pots were ranked in terms of quality with I representing the highest quality

and 6 the lowest quality. In addition, judges identified pots viewed

acceptable for purchase as a gift. Because plants were not pinched,

grown under shade cloth, or treated with growth regulators, quality estimates

probably represent a minimum for each treatment.

Pots from the aphid infested cages were judged completely unacceptable,

always ranking the poorest in quality and never being acceptable for purchase

as a gift. Pots from greenhouses where wasps were released from four

sites always ranked higher in quality than pots from greenhouses where

wasps were released from a single location.

Further, the percentages of pots deemed acceptable for gift-giving

from the 4-release point greenhouses were greater than the percentages

of gift quality pots never infested with aphids. Thus, biological

control is not only an effect method of aphid control, but facilitates

production of high quality potted crysanthemums. This biological

control releases technology, and the sampling program developed coincidentally,

are ready for final evaluation. During the 1998/99 fiscal year, sampling

validity and aphid biological control will be evaluated in commercial chrysanthemum

greenhouses in terms of pest control, economic feasibility, and grower

acceptance. Trials will be conducted in two sites and at two different

times of the year to assess the influence of cultural practices on the

sampling and biological control programs. The sampling program is

compatible with existing monitoring programs targeted at other key pests

and the biological control methods in two sites and at two different times

of the year to assess the influence of cultural practices on the sampling

and biological control programs. The sampling program is compatible

with existing monitoring programs targeted at other key pests and the biological

control methods evaluated will be applicable for natural enemy releases

targeted at other pests of floricultural crops.