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Insecticidal Controlled Atmosphere for Management of Sweetpotato Whitefly Progress Report — December 1995

Date

12/22/95

Title of Project Insecticidal Controlled Atmosphere for Management

of Silverleaf Whitefly

Institution where work is being conducted University of Massachusetts

Amount of Endowment Grant $14,174

Covering Period 2/95 to 1/96

Anticipated Date of Project Completion/Final Report 1/96

Individual(s) Conducting Project:

(List Project Leader First)

Dr. Susan S. Han - Title Assistant Professor

Telephone Number 413-545-5228

Insecticidal Controlled Atmosphere for Management of Silverleaf Whitefly

Susan S. Han

University of Massachusetts

Progress Report to the American Floral Endowment, 12/22/95

A. Project Objectives:

The main objective of the study is to investigate the potential use of

low-oxygen

atmospheres for control of silverleaf whitefly (Bemisia argentifolii)

on floricultural crops. The method

refers to reducing the levels of oxygen surrounding the plants. In

recent years, the use of elevated

CO2 or reduced 02 atmospheres to induce sublethal and lethal effects

on insects has provided an

excellent alternative to chemical fumigation for quarantine treatment

of imported edible crops. The

time required for 100% mortality of an insect depends on the species,

its developmental stage, and

the temperature and atmospheric composition during the treatment. Commodities

differ in their

susceptibility to CA and the recommendations for tile level of tolerance

to reduced 02 and/or

elevated CO2, varies (Ke and Kader, 1992). Floricultural crops can

tolerate higher levels of CO,

than most edible crops, perhaps due to the higher surface to volume

ratio (Joyce and Reid, 1985).

B. Results to date

Results from my laboratory have demonstrated that reduced 02 treatments

(<2 ppm

O2) are effective against both greenhouse and silverleaf whitefly.

Treatment time required for high

mortality rates depends on the developmental stage of the whitefly.

Adults and nymphs are sensitive

to the treatment where a 2 and 4 hr treatment at 20′C, respectively,

killed most of the insect. In

contrast, the egg and pupal stages are more resistant and required

an 8-hr treatment at 20′C to a

achieve high rate of mortality. The efficacy of the treatment increased

with an increased in

temperature from 20′C to 30′C. At 30′C, a treatment time of one-half

that of 20′C can be used to

achieve the same mortality rate.

We have evaluated the tolerance level of two cultivars

of geraniums

(’Red Orbit’and ‘Red Elite’) and two cultivars of coleus (Jazz Ruby’

and ‘Wizard Velvet) to tile

low-oxygen treatment. During the spring, seeds were sown and plants

were grown in the glasshouse

at the University of Massachusetts at Amherst. Plants were then treated

with a constant flow of air

(control) or with factory-mixed nitrogen gas (containing < 2 ppm

O2) at 200C for 0, 4, and 8 hr at

the 72-plug, 606 cell-pak, or 4″-pot stage. Following the gas treatment,

plants were transferred to a

larger container and were grown in the glasshouse for 4 weeks before

harvest. Data such as the

height, width, fresh weight and dry weight of the plants were collected

and analyzed statistically.

Results showed that of the two cultivars of geraniums tested, 8 hr

of low-oxygen treatment did not

affect the growth and develpment of the plants treated at the 6-pak

and 4″-pot stage. Geraniums

treated for 8 hr with low oxygen at the 72-plug stage were smaller

than those treated with air or 4-hr

low oxygen. The same studies repeated on two cultivars of coleus in

the summer showed that

treatments with low oxygen, up to 8 hr at 20′C, did not affect the

subsequent growth of the plants,

regardless of the stage of development. Results thus suggested that

the susceptibility of plants to the

low oxygen treatment depends on the developmental stages of the plants.

Casual observation

indicated that plants grown in the summer were less susceptible to

the low-oxygen treatment than

those grown in the spring perhaps owing to the higher light levels

in the summer. Further studies will

be conducted to confirm the observation.

C. Future plans

We are currently evaluating the insecticidal effects

of low-oxygen atmospheres on silverleaf whitefly when treated at 25′C.

In addition, we will

continue our investigations on the tolerance levels of various species

of annuals to the reduced-02

environment. D. Benefits for floral industry With the anticipated ban

of methyl bromide, a fumigant

widely used by the floricultural industry, finding a alternative means

to disinfest plant materials will

become a priority in the industry. The proposed method of reduced-02

insecticidal controlled

atmosphere would provide the greenhouse industry wit an environmentally-safe

way of controlling the

spread of whitefly through infested plant materials. The use of this

technique can be incorporated into

an Integrated Pest Management (IPM) program. Practice of such an approach

would drastically

reduce the amount of hazardous pesticides used, and would increase

profitability to the greenhouse

industry by reducing pesticide costs and increasing the quality of

the product.