Date Aug. 31, 1993

Title of Project Detection and Partial Characterization of Pelargonuim Viruses with Emphasis on

Pelargonium Flower Break Virus

Institution where work is being conducted The Ohio State University

Amount of Endowment Grant $ 15,000
Covering Period 1993 To 1996

Anticipated Date of Project Completion/Final Report Fall 1995

Individual(s) Conducting Project:

(List Project Leader First)

Dr. Stephen T. Nameth - Title Assoc. Professor

Telephone Number (614) 292-1375

Mr. Rob Fisher - Title Grad. Research Asst.

Detection and Partial Characterization of Pelargonium Viruses with Emphasis on Pelargonium Flower Break Virus

Stephen T. Nameth and Marie Anderson

Ohio State University

Progress Report to the American Floral Endowment, 8/31/93

A. Project Objectives:

1. Initiate a detailed on pelargonium flower break virus (PFBV) in an effort to

better characterize this virus.

2. To continue developing state-of-the-art methods of virus detection, identification

and characterization and to apply those methods to a detailed evaluation of

viruses or virus-like agents that infect geraniums.

B. Summary of Work Conducted:

PFBV has been purified, and the virus particles partially characterized. RNA extracted

from purified virus has been cloned and 85% sequenced. With the cooperation of the USDA antiserum has been produced to pelargonium

line pattern virus (PLPV). Work with tomato ringspot virus (TomRSV) has been put on hold in an effort to focus

on PFBV and PLPV.

C. Results to Date:

PFBV has been purified, partially characterized, cloned and 85% sequenced.

Antiserum has been made to PLPV and is in the process of being tested

against various isolates of PLPV. (A graduate student has been assigned to this

project.

D. Future Plans Covered by the Endowment Grant:

Work will continue on all objectives.

A research publication is being written on the results of the PFBV work.

E. Anticipated Benefits for Floral Industry:

With the cloning of PFBV a highly sensitive diagnostic probe will be possible,

also the possibility of coat protein mediated resistance to PFBV in geraniums

will be explored.

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