Detection and Partial Characterization of Pelargonium Viruses with Emphasis on Pelargonium Flower Break Virus Progress Report — March 1993
Date February 25, 1993
Title of Project Detection and Partial Characterization of Pelargonium Viruses with Emphasis on Pelargonium Flower Break Virus
Institution where work is being conducted The Ohio State University
Amount of Endowment Grant $ 15,000.00
Covering Period 1993 to 1996
Anticipated Date of Project Completion/Final Report Fall 1995
Individual(s) Conducting Project:
(List Project Leader First)
Dr. Stephen T. Nameth - Title Associate Professor
Telephone Number (614) 292-1375
Ms. Marie Anderson - Title Graduate Research Associate
Detection and Partial Characterization of Pelargonium Viruses with Emphasis on Pelargonium Flower Break Virus
Stephen T. Nameth and Marie Anderson
Ohio State University
- A. Project Objectives:
- 1. Initiate a detalied study on pelargonium flower break virus (PFBV) in an effort to better
characterize this virus.
- 2. To continue developing state-of-the-art methods of virus detection, identification and
characterization and to apply those methods to a detailed evaluation of viruses or virus-like
agents that infect geranium.
- 2. To continue developing state-of-the-art methods of virus detection, identification and
- B. Summary of Work Conducted:
- Pelargonium flower break virus has been purified in quantity from Chenopodium tissue. RNA has
been extracted and is in the process or being cloned. Detailed work has started with Pelargonium
line pattern/ring pattern virus in an effort to compare the U.S. isolates against those from Europe.
Purification of tomato ringspot virus has started with a geranium isolate of TomRSV.
- C. Results to Date:
- Pel. FBV has been purified and RNA extracted. Plants are infected with U.S. and European
isolates of Pel Line Pattern/Ring Pattern Virus. Purification of TomRSV has started.
- D. Future Plans Covered by the Endowment Grant:
- Work will continue on all objectives as outlined.
- E. Anticipated Benefits for Floral Industry:
- More sensitive and more accurate diagnostic techniques will be developed allowing for earlier
detection of pelargonium viruses.
