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The Effect of Modifying the Microenvironment Among Geranium Stock Plants Within a Greenhouse on Botrytis Blight and Sporulation of Botrytis Cinerea Progress Report — September 1992

Date 8/28/92

Title of Project The Effect of Modifying the Microenvironment Among Geranium Stock Plants

Within a Greenhouse on Botrytis Blight and Sporulation of Botrytis Cinerea

Institution where work is being conducted Michigan State University

Amount of Endowment Grant $25,000
Covering Period 1/1/92 to 12/31/92

Anticipated Date of Project Completion/Final Report 12/31/93

Individual(s) Conducting Project: Mary K. Hausbeck

(List Project Leader First)

Michigan State University – Title Visiting Assistant Prof.

Telephone Number (517) 355-4534

The Effect of Modifying the Microenvironment Among Geranium Stock Plants on Botrytis Blightand Sporulation of Botrytis Cinerea

Mary K. Hausbeck

Michigan State University

Progress Report to the American Floral Endowment, 8/28/92
A. Project Objectives:
The primary objective addressed by this progressreport relates to the production of pathogen-free planting stock.

B. Summary of Work Conducted:
In the previous progress report it was demonstratedthat modifying the environment among stock plants using forced heated

air can negatively impact the occurrence of leaf blight among cuttings

taken from those stock plants. In that first study, an average of 74%

of the leaves became blighted due to Botrytis when cuttings were taken

from control plants grown in an environment that was not modified and

exposed to optimum conditions for disease development. In contrast,

only 26% of the leaves became blighted when cuttings were taken from

geranium stock plants exposed to forced heated air and treated in a

similar manner. Most recently upon further analysis, it was determined

based on the monitoring of environmental conditions, that prior to

removing the cuttings, the stock plants in the modified environment did

not experience leaf wetness whereas the plants in the environment that

was not modified experienced nearly 3 1/2 days of leaf wetness. These

wet conditions prior to the removal of cuttings from the stock plants

apparently resulted in conditions favorable for infection of the stock

plants by Botrytis and subsequent sporulation. Previous work conducted

in connection with this study showed that removal of cuttings from stock

plants in an environment that was not modified resulted in “spore

showers” providing inoculum for cuttings removed these plants. Once the

cuttings are placed in an appropriate environment for Botrytis

development (i.e. wet, high relative humidity) these spores germinate

resulting in leaf blight. This initial study has now been repeated

three times and the trend has been similar in that there was less leaf

blight in cuttings removed from stock plants grown in the modified

environment versus those grown in an environment that was not modified.

The amount of leaf blight occurring in the cuttings from plants grown in

the environment that was not modified varied according to the hours of

leaf wetness experienced by the stock plants just prior to harvest.

It is clear from these studies that environmental manipulation of the

stock plant growing area has significant implications for disease

management in the next step of the production chain which involves the

propagation greenhouse. This environmental manipulation reduces the

sporulation on the stock plants thereby reducing the inoculum that

occurs on the cuttings prior to their placement in the propagation house

which is wet and humid and conducive to Botrytis development. This

information will be helpful for all crops that are grown in a stock

plant and propagation misting system and is especially critical given

the continuing reports of resistance of Botrytis to commonly used

fungicides. Future plans include expanding these studies to further

understand the environmental parameters necessary for disease

development.

C. Anticipated Benefits for Floral Industry:
The environmental parametersnecessary to deter Botrytis blight will be formulated into a forecasting

system to provide growers with an economical disease management tool

that improves plant quality by reducing disease and pesticide sprays.