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Plant Resistance as a Part of Integrated Pest Management for Whiteflies on Floral Crops Progress Report — September 1992

Date 23 August 1992

Title of Project Plant Resistance as Part of IPM for Whiteflies on Floral Crops

Institution where work is being conducted Cornell University

Amount of Endowment Grant $27,000
Covering Period 1/92 to 12/92

Anticipated Date of Project Completion/Final Report one year hence

Individual(s) Conducting Project:

(List Project Leader First)

Dr. John P. Sanderson - Title Asst. Professor of Entomology

Telephone Number (607) 255-5419

Plant Resistance as part of IPM for Whiteflies on Floral Crops

John P. Sanderson

Cornell University

Progress Report to the American Floral Endowment, 8/23/92

A. Project Objectives:

1. Screen cultivars of several important floral crops for resistance to whiteflies.
2. Investigate the degree to which crop fertilizing practices affect whitefly biology (and

perhaps that of other pests), and determine if pest problems can be reduced by modifying

crop fertilizer programs without sacrificing crop quality.

3. Compare the efficacy of insecticides against whiteflies on resistant and susceptible

cultivars.

4. Compare the effectiveness of whitefly parasitoids on selected crop cultivars.

B. Summary of Work Conducted/Results to Date:

[Note: An article published in the Proceedings of the SAF 8th Conf. on Insect &

Disease Mgt. (Feb., 1992, in Orlando, FL) presents an overview of much of our

progress.]

Objective 1. We are in the process of identifying plant factors that influence whitefly

numbers. We are currently testing our suspicion that trichome (leaf hair) characteristics are

important. Trichome numbers per sq. mm vary significantly among the 26 poinsettia

cultivars tested. When whiteflies are provided a choice among cultivars, whitefly numbers

seem to be somewhat positively correlated with those cultivars that have higher trichome

densities. To test whether trichomes influence egg production, we tried several ways to

remove leaf trichomes without damaging the leaf, but were not successful. We are now

testing our hunch by looking at sweetpotato whitefly egg production in choice and no-

choice tests within and between groups of cultivars that have similar trichome densities.

We predict that egg production will be greatest on the cultivars with the most trichomes.

Objective 2. My graduate student, Dorothy Boorse, has been responsible for

examining the influence of nitrogen fertilizer on whitefly biology for her M.S. thesis. She

has completed her last set of experiments and is currently analyzing her results. Generally

speaking, within the range of nitrogen fertilizer rates that are commercially typical, she has

seen relatively minor affects on sweetpotato whitefly egg production and developmental

time. We will provide a full report when her results are fully analyzed.

Objective 3. Our results thus far indicate that the most important effect that cultivar has

on chemical effectiveness is whether a cultivar’s plant architecture allows for effective

coverage and canopy penetration. Besides our on-going insecticide studies for sweetpotato

whitefly control, we recently evaluated the performance of two aerosol insecticide

formulations among several poinsettia cultivars. We Were interested in how cultivar

characteristics such as leaf shape, size, and canopy density would affect the effectiveness

of these aerosols against immature whiteflies (nymphs). One insecticide was a typical

nerve poison (PT 1200 Orthene plus PT 1800 Attain); the other was an insect growth

regulator (Preclude). No difference in mortality due to cultivar was noticed, but this was

largely because none of the insecticides were very effective in killing the nymphs. We

suspect that the aerosol formulations were not contacting the numphs where they occur on

the leaf undersurfaces, thus we saw no difference in nymph mortality among cultivars.

The mixture of PT 1200 Othene plus PT 1800 Attain was effective in killing adult

whiteflies, however, although more than 10 applications at 4 day intervals were necessary

to achieve adequate control of the serious whitefly infestation that was present at the start of

the trial. This information is important in order to properly know how to use these aerosol

insecticides and what to expect of them, We have additional studies planned or underway

with seven new insecticides or new insecticide formulations.

Objective 4. As noted in Objective 1, trichome density may affect parasitoid

performance as well as whitefly numbers. The pattern of percent parasitism by Encarsia

formosa differed significantly among seven poinsettia cultivars in a previous study,

indicating a potential cultivar effect on the effectiveness of the wasps. We are examining

the impact of trichomes on parasitoid performance in conjunction with the work mentioned

in Objective 1. We are also beginning some evaluations of a new parasitoid species for

sweetpotato whitefly in these studies.

C. Future Plans Covered by the Endowment Grant:

Objective 1. We will continue to evaluate poinsettias and other floral crops for

resistance to whiteflies. We will evaluate the importance of leaf trichome density (”leaf

hairiness”) on SPWF numbers on poinsettia in the short term, and other plant

characterisitics (e.g., sap content) in the future.

Objective 2. This objective will be completed soon, once the results are analyzed and

interpreted.

Objective 3. We will continue our screening of potential insecticides for whitefly

control, including an analysis of cultivar effect on insecticide performance.

Objective 4. We suspect that leaf trichome density influences parasitoid searching

efficiency and effectiveness. We plan to examine this experimentally in conjunction with

Objective 1, as well as with small scale field tests.

D. Anticipated Benefits for Floral Industry:

This research will aid in whitefly control with less pesticides, provide

growers with more “least toxic” pest control strategies, and avoid problems associated with

the overuse of insecticides. Knowledge of plant resistance factors to whiteflies in

floricultural crops can be a valuable tool in breeding programs. For example, if trichomes

are important in plant resistance to whiteflies, this trait could be modified via breeding

programs. Screening popular cultivars for plant resistance can give growers information

on which cultivars should be carefully inspected and monitored for pest populations, or

those cultivars on which chemical or biological control will be more effective.

A side benefit of this work is our ability at Cornell to incorporate pertinent results into

our New York State Greenhouse IPM effort, thereby testing it in the hands of growers and

eventually implementing it. From a scientific standpoint, these studies may be useful to

other scientists involved in plant breeding, chemical control, biological control, and IPM.

For example, our work with the influence of cultivar on whitefly parasites will complement

biological control studies underway by other entomologists. Our results may also be

directly applicable to plant resistance studies of other greenhouse pests.