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Blue Gum Psyllid Biological Control 1992 Proposal

Blue Gum Psyllid Biological Control

Dr. Donald Dahlsten

University of California

1992

X. A. SUMMARY:

The objective of this project is to study the biology of the blue gum

psyllid, Ctenarytaina eucalypti, in northern and southern California Eucalyptus

plantations. Secondly, we will develop a sampling program so that populations

of the blue gum psyllid can be monitored and the effect of the natural

enemies on the blue gum psyllid quantified. The final objective is to search

for natural enemies of the blue gum psyllid in its native home, Australia,

import them into quarantine facilities at our laboratory in Albany and

eventually release the natural enemies in northern and southern California

Eucalyptus plantations.

If the introduction of the natural enemies of the blue gum psyllid are

successful, costly insecticide spray applications can be avoided and this

will save the industry large sums of money. The beauty of a successful

biological control is that it is economical, permanent and safe. If the

natural enemies are only partially successful, then they will be incorporated

into an integrated pest management program for the blue gum psyllid.

X. B. DETAILED PROPOSAL.

Introduction and Background Information.

The blue gum psyllid, Ctenarytaina eucalypti, is a new pest in California.

It was first found in eucalyptus nursery growing grounds in Monterey County

in January, 1991. Since the original find near Prunedale, it has been collected

at numerous sites in Monterey County, from Watsonville south to Salinas.

In addition to Monterey County, Ray Gill (1991), CDFA taxonomist, has Identified

C. eucalypti from material collected in Santa Cruz, Contra Costa, Alameda,

Solano, Santa Clara, San Luis Obispo, Los Angeles, Orange and Alarneda,

counties, and it is thought to be widespread in other San Francisco Bay

Area counties, the Los Angeles basin, as well as extending into the Sacramento

Valley. The blue gum psyllid is native to Australia, and prior to its appearance

in California, has been accidentally introduced to other regions of the

world where eucalyptus is grown. These areas include New Zealand, Sri Lanka,

and South Africa. In Europe, it was first found in Great Britain in 1922,

Portugal in 1971 and Spain in 1972. Most of the information regarding the

biology of the blue gum psyllid and it damage comes frorn studies done

in Europe (Azavedo and Figo, 1979; Cadahia, 1980, 1986; Cadahia and Ruperez,

1979; Martinez, 1983).

The psyllid feeds and develops on the juvenile foliage of several eucalyptus

species, some of which are grown primarily for use In the floral trade,

In addition, it attacks eucalyptus species that are utilized in landscaping.

Acute damage caused by high psyllid densities include inhibition of

new shoot formation, distortion of the shape of new foliage, and spoiled

plant appearance as a result of the growth of black sooty molds on the

honeydew and white flocculate secretions produced by the immature. Because

this is such a recent problem, chronic effects of sustained high psyllid

densities can only be speculated as being severely weakened plants, exhibiting

poor growth characteristics and consequently lowered economic value. Insecticide

intervention has proven to be costly and for the most part ineffective,

in addition to contributing to potentially harmful environmental impacts.

This coupled with the fact that psyllid populations are unchecked by any

of California’s native natural enemies make the blue gum psyllid a good

candidate for the classical biological control approach to its control

in California.

Review of Significant Literature.

The lack of a substantial body of literature regarding the blue gum

psyllid in Australia is taken as an indication that it is not of sufficient

economic importance to attract the attention of Australian entomologists

and is most likely under control by natural enemies there. This is quite

similar to our experiences in each of above mentioned biological control

projects. In fact, in the case of the Acacia psyllid, prior to our work,

Australian entomologists were not even aware that it occurred in Australia,

lot alone that its populations were being regulated by the coccinellid.

Almost all of the research on the blue gum Psyllid comes from studies conducted

in Europe. Most encouraging in this regard is a reference made, by Frank

Wilson (1963) on Australian beneficial insects in which he states that

“…in New Zealand, where it infests Eucalyptus globulus. A eulophid parasite,

which apparently reached New Zealand accidentally at the same time, controls

the psyllid.” The only other reference we have found that addresses the

blue gum psyllid in Australia is that of F. David Morgan (1984) where he

states that “The insect is very common but rarely develops outbreaks…”

and “It is heavily parasitized in the 5th instar and predators, particulary

honey-eaters, have been observed feeding upon nymphs and adults.

Objectives.

1. Study the biology of the blue gum psyllid in northern and southern

California Eucalyptus plantantions.

2. Develop a sampling procedure for the blue gum psyllid and its natural

enemies.

3. Import natural enemies of the blue gum psyllid from Australia.

4. Release and evaluate the efficacy of natural enemies of the blue

gum psyllid in California Eucalyptus plantations.

5. Develop a biological control program for the blue gum psyllid.

Materials and Methods.

Our approach will be to conduct a search for the eulophid and other

natural enemies of the blue gum in Australia, beginning in September, 1991.

Collections of potontial natural enemies will be shipped by air back to

our USDA/CDFA approved quarantine facilities located at the University

of California, Berkeley, Division of Biological Control in Albany, California.

We also have access to the USDA quarantine greenhouse facility, located

at the USDA Western Region laboratories, Albany, California. In quarantine,

the shipments will be screened to exclude potential harmful organisms,

i.e. hyperparasites, and reared for at least one generation on pure colonies

of blue gum psyllid before being released to the our insectary for large

scale rearing. This is one of required steps of Federal and State protocols

required prior to the release of organisms in California.

In preparation for the release phase of the project, we will setup study/release

sites with grower-cooperators in Monterey County, in northern California,

and begin to systematically monitor psyllid populations, Similar study/release

sites will also be established with grower-cooperators in southern California,

This information will allow us to document the subsequent effect the introduced

natural enemies have in controlling blue gum psyllid populations.

Once the introduced natural enemies have colonized the original release

sites and are considered to be permanent residents, these sites will be

used as nursery sites from which the introduced enemies will be distributed

to other areas of the State.

Sampling and biology studies will provide information needed to evaluate

the success of the introduced natural enemies and assess the need for further

importations.

Over the past 20 years, we have had considerable experience and success

with the introduction of natural enemies against pests of ornamental plants

in California. Beginning in 1972, we successfully introduced a coccinellid

from Australia against the Acacia psyllid; in 1978, we established three

encyrtid parasites from South Africa against two species of iceplant scales;

and more recently, in 1988, we successfully imported a eulophid parasite

from Chile against the Pepper tree psyllid. The key personnel, Drs. Kenneth

Hagen and Richard Tassan (UCB), involved with these efforts will be available

to contribute their full expertise: and energies towards the blue gum psyllid

biological control effort. In addition, Drs. Robert Luck (UCR) and Leo

Caltagirone (UCB) will be in Australia to assist with the exploration for

psyllid natural enemies. Australia entomologists, Dr, Keith Taylor of CSIRO

in Canbera and Dr. Gary Taylor of the University of Adelaide in South Australia,

both leading authorities on psyllids have agreed to collaborate with us

on this project. We will also be working closely with UC Farm Advisors,

Bill Chanty, Steve Tjosvold and Karen Robb throughout the project.

Time Table.

1991

a. Establish greenhouse/insectary colonies of blue gum psyllid,

b. Develop sampling procedures.

c. Begin studies of psyllid biology in field and laboratory and establish

damage thresholds.

d. Conduct initial exploration for psyllid natural enemies in Australia,

and import into California.

1992

a. Meet protocols for release of imported natural enemies from quarantine.

b. Establish laboratory colonies of natural enemies.

c. Conduct preliminary studies of natural enemy biologies.

d. Make initial natural enemy releases and monitor for field colonization.

e. Expand sampling program to include natural enemy impact.

f. Expand number of release sites in both northern and southern California.

g. Publish results.

1993-1994

a. Continue sampling program to determine natural enemy impact.

b. Expand natural enemy distribution statewide.

c. Complete detailed psyllid and natural enemy biology studies.

d. Evaluate success of biological control project and assess need for

further work.

e. Publish results.

Facilities and Equipment Available.

All of the facilities and equipment are available at the Division of

Biological Control’s Laboratory in Albany, including an officially approved

quarantine facility and a large insectary for rearing natural enemies.

Literature Cited.

Azevedo, F. and M, L. Figo 1979, Ctenarytaina eucalypti Mask (Homoptera,

Psyllidae). Bol. Serv, Plagas. 5:41-46,

Cadahia, D. 1980. Proximidad de dos nitevos enemigos de los Eucalyptus

en Espana. Bol. Serv. Plagas. 6:165-192,

Cadahia, D, 1986, Importance des insectes ravageurs de l’eucalyptus

en region mediterraneenne. Bulletin OEPP. 16:265-283.

Cadahia, D. and A. Ruperez. 1979. Reparticion de Crenarytaina eucalypti

Mask. en Espana, Bol, Serv. Plagas. 5:55-58.

Gill, R. 1991. California Plant Pest and Disease Report. California

Department of Food and Agriculture. 10:5-7.

Martinez, M, 1983. Possibilite d’introduction en France de deux Inseotes

ravageurs specifiquos des Eucalyptus: Phoracantha semipunctata (Col. Cerambycidael

et Ctenaryfaina eucalypti [Hom. Psyllidae), L’Entomologiste, 39:53-57.

Morgan, F. D. 1984. Handbook of the Flora and Fauna of South Australia,

Psylloldea of South Australia. Government Printer, South Australia. 130pp,

Taylor, K. L. 1990. The tribe Ctenarytainini (Hemiptera: Psylloidea):

A key to the known Australian genera, with new species and two new genera.

Invertebr. Taxon. 4:95-121.

Wilson, F. 1963. Australia as a source of beneficial insects for biological

control. Technical Communication No. 3, Commonwealth Institute of Biological

Control, Curepe, Trinidad. 28pp. Natural Enemies Associated with Blue Gum

Psyllid

PREDATORS:

Syrphidae Melliscaeva cinctellus Zett. — Europe Pipizella sp. — Europe

PARASITES:

Eulophidae unidentified species — New Zealand, thought to be originally

from Australia

3 – YEAR BUDGET

(9/2/91 – 6/31/94) YEAR 1

9/1/91 -8/31/92 SALARIES:

GSR @ 50% x 12 mos. $12,432 Lab Assistant I @ 25% X 12 4,740 $17,172

BENEFITS:

12,432 @ 2.84% 353 4,740 @ 1.8% 85 $438

MISC. S & E:

Misc. rearing supplies, potted plants, etc. $1,000

EQUIPMENT:

3 temperature loggers @ $1,500 ea $4,500 TRAVEL

Air travel to So. California (3 roundtrip tickets); Auto travel to sample

in So. and No. California $5,000

TOTAL (Year 1) $28,110

YEAR 2

9/1/92 -8/31/93

SALARIES:

GSR @ 50% x 12 mos. $13,054 Lab Asst. I @ 25% x 12 mos. 4,977 $18,031

BENEFITS: 461 S & E 1,000

TRAVEL: 5,000

TOTAL (Year 2) $24,492

YEAR-3

9/1/93 – 8/31/94

SALARIES:

GSR @ 60% x 12 MOS. $13,707 Lab Asst I @ 25% x 12 mos. 5,226 $18,933

BENEFITS: 456 S & E: 1,000

TRAVEL 5,000

TOTAL (Year 3) $25,389

JUSTIFICATION:

We will need the equivalent of one full-time person to rear parasites

and handle the quarantine operations. This person will here the sampling

in northern California. We will cooperate with William Chaney In Salinas

and Steve Tjosvald In Watsonville. We will need a part-time person under

the direction of Dr. Robert Luck at V. C. Riverside to do sampling, parasite

release and monitoring. In southern California, we will be cooperating

with Dr. Karen Robb of U.C. Extension In San Diego and Dr. Rich Cowles

at U.C. Riverside.

X. C. PROJECT LEADER QUALIFICATIONS

Donald L. Dahlsten:

I have been teaching and conducting research on the biological control

of forest and urban tree insects for 28 years. I have published over 150

research papers and other articles on insect ecology, forest entomology,

biological control, integrated pest management, and urban tree pest management.

(See attached bio- bibliography.)

BIOGRAPHICAL INFORMATION SHEET

Donald L. Dahlsten

Name: Donald Lee Dahlston, born 8 December 1933, Clay Center, Nebraska

Title: Professor of Entomology, Department of Entomological Sciences,

Division of Biological Control, University of California, Berkeley

and Entomologist in the Agricultural Experiment Station

Education: Graduated from Hollywood High School, Los Angelos. CA.,

Feb. 1952

B.S. University of California, Davis, 1966

M.S. University of California, Berkeley, 1960

Ph.D. University of California, Berkeley, 1963

Background:

1. Research Assistant In Enlomology, University of California, Berkeley,

Feb. 1959 to July 1959

2. Laboratory Tech. II in Entomology, University of California, Berkeley,

July 1959 to Sept. 1962

3. Assist. Professor of Zoology, Los Angeles State College, Los Angeles,

Sept. 1962 to Sept. 1963

4. Assistant Entomologist, University of California, Berkeley, October

1963 to July 1965

5. Lecturer In Entomology, UCS, July 1965 to July 1968

6. Assistant Professor of Entomology, UCB, July 1968 to July 1969

7. Associate Professor of Entomology, UCB, July 1969 to 1974

8. Professor of Entomology, UCB, July 1974 to present

(Chair, Division of Biological Control, 1981-1988)

Research Interests:

Biological control of forest insects. Side-effects of chemical insecticides

in forest communities. Population

dynamics of bark beetles and forest defoliators. Biology of cavity-nesting

birds and impact on forest insect

populations. Human activity and the creation of entomological problems

in montane environments.

Biological control of posts of urban trees and shrubs.

Honors and Awards:

American Institute Biological Sciences Visiting Professor, 1970-71;

Mellon Visiting Lecturer In Entomology,

Yale School of Forestry and Environmental Studies, 1980; Member of

Forestry Technology Exchange

Team, People’s Republic of China, 1981; Delegation leader of Team on

Integrated Pest Management In

Forestry to Peoples Republic of China 1982. Selected by University

of California, Berkeley to make

presentation on topic of biological control to Professor Zhao Zlyang

of PRC during his visit in 1984.

Memberships:

American Associate Advancement Sciences; American Institute Biological

Science; American Museum

Natural History; California Forest Post Control Council; Ecological

Society of America; Entomological

Society of Canada; National Audubon Society, Northern California Committee

for Environmental

Information; Pacific Coast Entomological Society: Sigma XI, Society

of American Foresters; Western Forest

Insect Work Conference; Sierra Club; Scientists’ Institute for Public

Information; XI Sigma PI, Theta Chapter.

150 publications, including the most recent, as follows:

RECENT PUBLICATIONS

1987. Drelstadl, S, H. and D. L. Dahlsten. Report on Reports. Pesticide

Resistance: Strategies and

Tactics for Management, U. S. National Research Council, Environment

29(3): 25-27.

1987 Miller, M. C., J. C. Moser, M. McGregor, J.C. Gregoire, M. Salsler,

D. L. Dahlsten, and R, A. Werner.

Potential for biological control of native North American Dendroctonus

beetles (Coleoptera:Scolytidae).

Annals Entomol. Soc. Amer. 80(3): 417-428.

1987. Drelstadt, S. H., D. L. Dahlsten and K. S. Hagen. Integrated

pest management of tulip tree aphids,

pp.35-37. In R. Venne et al, editor. Minimal maintenance landscaping.

A report of the Elvenia J. Slosson

Fund for Ornamental Horticulture: 1983-1986. Univ. of Calif., Div.

of Agric. and Nat. Resources. 47 pp.

1987. Dahlsten, D. L. and S. H. Drelstadt. Elm leaf beetle IPM research

update, Growing Points – Central

Coast Counties 24(11): 3-4.

1987, Hajek, A. E. and D. L. Dahlsten. Behavioral Interactions between

three birch aphid species and

Adalia bipunctato larvae. Entomologla Experimentalls et Applicata 45:

81-87.

1987. Hajek, A.E. and D. L. Dahlsten, The exotic aphids (Homoptera:

Drepanosiphidae) on ornamental

birch in northern California. Pan-Pacific Entomol. 63(4): 319-323.

1987, Clair, D. J., D. L. Dahlsten and E. R. Hart. Rearing Tetrastichus

gallerucae (Hymenoptera:

Eulophldae) for biological control of the elm leaf beetle, Xanthogaleruca

luteola. Entomophaga 32(6): 467-

461.

1988. Hajek, A. E, and D. L. Dahlsten. Distribution and dynamics of

aphid (Homoptera: Drepanosiphidae)

populations on Betula pendula in northern California. Hilgardia 56(1):1-33.

1988. Clair, D. J., D. L. Dahlsten, and S. H. Drelstadt. Biological

control of the elm leaf beetle,

Xanthogaleruca luteola in California – A case study. Vol. 2, pp, 497

a 504. In Global Perspectives on

Agroecology and Sustainable Agricultural Systems (P. Allen and D. van

Dusen, eds). Proc. 6th

International IFOAM Conf., Santa Cruz, California, August 18-21, 1986.

2 vols., 730 pp.

1988. Drelstadt, S. H. and D. L. Dahlsten. Tuliptree aphid honeydew

management. J. Arboriculture 14(9):

209-214.

1989. Drelstadt, S. H. and D. L. Dahlsten. Gypsy moth eradication in

Pacific Coast states: History and

evaluation. Bull. Ent. Soc. Amer. 35(2): 13-19.

1989. Dahlsten, D, L, and R. Garcia, editors. Eradication of Exotic

Pests: Analysis with Case Histories.

Yale University Press, New Haven, Connecticut. 296 pp.

1989. Dahlsten, D. L., R. Garcia, and H. Lorraine. Eradication as a

pest management tool: concepts and

contexts. pp. 3-15. In Eradication of Exotic Posts: Analysis with Case

Histories (D. L. Dahlsten and R.

Garcia, eds). Yale University Press, New Haven, Connecticut. 296 pp.

1989. Dahlsten, D. L. and M. C. Whitmore. Potential for biological

control of Dendroctonus and Ips bark

beetles: the case for and against biological control of bark beetles,

pp. 3-19, In Potential for Biological

Control of Dendroctonus and Ips Bark Beetles (D. L. Kulhavy and M.

C. Miller, eds.). Center for Applied

Studies, School of Forestry, Stephen F. Austin State University, Nacagdoches,

Texas. 265 pp.

1989. Berisford, C. W. and D. L. Dahlsten. Biological control of Ips

grandicollis (Elchhoff) (Coleoptera:

Scolytidae) In Australla–a preliminary evaluation, pp, 81-93, In Potential

for Biological Control of

Dendroctonus and Ips Bark Beetles (D. L. Kulhavy and M. C. Miller,

ads.), Center for Applied Studies,

School of Forestry, Stephen F. Austin State University, Nacagdoches,

Texas. 265 pp.

Morrison, M. L., R. L. Heald, and D, L. Dahlston. Can incense-cedar

be managed for birds? Western J. of

Applied Forestry 5(1): 26-30.

1990. Drelstach, S. H. and D. L. Dahlsten. Relationships of temperature

to elm leaf beetle (Coleoptera:

Chrysomelidae) development and damage in the field. J. Econ. Entomol.

83(3): 837-841.

1990. Sower, L. L., J. M. Wenz, D. L. Dahlsten and G. E. Daterman.

Field testing of the pheromone

disruption on pre-outbreak populations of Douglas-fir tussock moth

(Lepidoptera: Lymantrildae). J. Econ.

Entomol. 83(4): 1487-1491.

1990. Drelstadt, S. H. and D. L. Dahlsten, Insecticide bark bands and

control of the elm leaf beetle

(Coleoptera: Chrysomelldae) In Northern California, J. Econ. Entomol,

63(4): 1495-1498.

1990. Daane. K. M., D. L. Dahlsten and S. H. Drelstadt. Effects of

Mediterranean fruit fly malathion bait

spray on the longevity and oviposition of parasitoids of the linden

and tulip tree aphids (Homoptera:

Aphididae). Environ. Entomol. 19(4): 1130-1134.

1990. Dahlstan, D. L., D. L. Rowney, W. A. Copper, S. M, Taft and J.

M, Wenz. Long-term population

studies of the Douglas-fir tussock moth in California, pp. 45-58. Ln:

Population Dynamics of Forest Insects

(A D, Watt, S, R. Leather, M. D. Hunter and N. A. C. Kidd, eds) Intercept

Ltd, Andover, England. 408pp.

1990. Dahlsten, D. L,, A. E. Halek and M. Wilson, Scientific Note.

Host specificity of two shade tree aphid

parasitoids, Pan-Pacific Entomologist 66(4):322-323,

1990. Drelstadt, S, H. and D. L. Dahlsten. Distribution and abundance

of Erynniopsis antennata (Diptera:

Tachinidae) and Tetrastichus brevistigma (Hymenoptera: Eulophidae),

two introduced elm leaf beetle

parasitolds in northern California. Entomophaga 35(4): 627-536.

1991. Dralstadt, S. H., D. L. Dahlsten, D. L. Rowney, S. M. Tait, G.

Y. Yokota and W. A. Copper. Treatment

of destructive elm leaf beetle should be timed by temperature. Calif,

Agric, 45(2): 23-25.

1991. Dahlstan, D. L, and S. H. Drelstadt. Forest post management sociopolitics,

Forest Ecology and

Management 39 (1-4): 289-297.

1991. Dahlsten, D. L. Dutch elm dispose in California: Control and

eradication, pp. 35-39. In Does the

Elm Have a Future In Australia. (T. E, Arthur and J. D. Hitchmaugh,

eds.) Proceedings of a seminar held at

Victorian College of Agriculture and Horticulture, Brunlay, 17 May

1990. 59 pp.